產(chǎn)品英文名稱:Rat C-peptide ELISA Kit 產(chǎn)品中文名稱:大鼠C-肽ELISA檢測試劑盒
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§ 檢測原理:ALPCO 大鼠C-肽 ELISA設(shè)計(jì)用于檢測大鼠血清中C-肽含量。ALPCO大鼠C-肽ELISA是一個(gè)夾層式免疫分析試劑盒。96孔微型板涂有一層C-肽特異性單克隆抗體。檢測分析使用有機(jī)合成C-肽蛋白分子量標(biāo)定。將標(biāo)準(zhǔn)品,對照物和樣品加到孔中和配體物混合。后放微孔板搖床上溫育,700-900rpm。*次溫育結(jié)束,用清洗緩沖液清洗小孔并吸干。加TMB作用底物,第二次溫育,700-900rpm。第二次溫育完成,加終止液,調(diào)節(jié)分光光度計(jì)到450nm測定光密度(O.D.)。由于光密度與樣品中C-肽含量成比例,所以可以根據(jù)O.D.值計(jì)算出C-肽含量。
§ 檢測過程:提前從冰箱中取出試劑盒,平衡至室溫→各移取10µl標(biāo)準(zhǔn)品,對照品和樣品到每個(gè)小孔中→移取100µl結(jié)合物到每孔中(配制方法參照說明書)→密封微孔板,放在微孔板搖床上室溫溫育2小時(shí),700-900rpm→清空小孔,350µl清洗緩沖液清洗三次(每次清洗具體方法不一樣,參照說明書)→每孔添加100µlTMB作用底物→密封微孔板,放在微孔板搖床上室溫溫育10分鐘,700-900rpm→每孔添加100µl反應(yīng)終止液,輕搖混合,清除氣泡再準(zhǔn)備下一步操作→放在微孔板讀數(shù)器,調(diào)節(jié)分光光度計(jì)到450nm讀數(shù)(30分鐘內(nèi))
ALPCO RAT C-peptide ELISA kit英文簡述: The ALPCO Rat C-peptide ELISA kit quantifies the C-peptide protein products of the rat I and rat II proinsulin genes. Utilizing a dual-monoclonal antibody sandwich format, bi-level control set, and 96-well microplate comprising removable strips, a single Rat C-peptide ELISA kit has the performance characteristics and flexibility necessary to confidently measure up to 40 samples in duplicate. The shelf-life of the components and the resealable microplate pouch allow for convenient storage for future use if the entire kit is not needed at one time.
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